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The Tridosha theory is a fundamental concept in Ayurveda that explains physiological function and individual health through the interplay of Vata, Pitta, and Kapha. Vata is traditionally associated with movement, communication, and nervous system functions; Pitta with digestion, metabolism, and transformation; and Kapha with structure, stability, and lubrication. According to Ayurvedic principles, the balance and interaction of these doshas influence individual constitution, health, and disease susceptibility. Clinical applications include assessing Prakriti, understanding symptom patterns, and guiding personalized dietary, lifestyle, and therapeutic recommendations. Exploring the Tridosha framework alongside contemporary physiological knowledge can support a clearer understanding of traditional clinical practice, while evidence-based evaluation remains essential when assessing diagnostic validity, treatment efficacy, and patient safety.